Yeast Ranching notes

These are some notes I took when the owner/brewer of the soon-to-be-opened Kelsey Creek Brewing gave a seminar on yeast ranching to some members of the Lake County Homebrewers club.

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To grow and cultivate (aka “ranch”) yeast you want clean clear wort with the trub precipitated out to mix with agar to put on the petri dishes. You will also need inoculation loops and  inoculation needles.

Required for yeast ranching:

Process:

Sprinkle agar over the top of the cooled wort. Do not dump the agar rather sprinkle in around (7g/250ml). No need to stir. Sanitation is not critical yet, since it will go into a pressure cooker which serves as an autoclave. Be sure to add nutrient to your wort.
After it has been used, every time you touch something in the pressure cooker you spray it with isopropyl 70 alcohol.

Pour the agar & wort solution using the “pacman” technique
Make sure the agar sets up ~45min before flipping over to lessen the condensation

Place a drop of diluted yeast solution on the dish with the agar. Then remember the spot and drop sterile saline on the yeast’s spot.

Then use the needle to drag the drop to streak it.

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Nice to haves:

  • Hemocytometer (local veterinary offices may have these lying around)
  • microscope

To check yeast viability a bulb flask is used (9 ml of sterile and 1 ml of yeast and 1 drop of methylene blue) and .0001 ml of of solution is placed on hemocytometer slide

Resources:

This Week on Brew Disasters: Off flavors-Banana & Clove in the Little Sumpin’ Sumpin’ clone

The Little Sumpin' Sumpin' Ale clone looking very cloney.

Last time on Brew Disasters we were trying to clone Lagunitas Brewing’s Little Sumpin’ Sumpin’ Al eand had several problems right out of the gate: Instead of a desired mash temp of 150F, the mash temp was 170F! And, the pre-boil specific gravity came in low at 1.043. That was a full 12 points below the 1.055 that was our target.

Well, it’s difficult for us to tell if the cooling of the grain from 170F to 149.5F succeeded. But, the adding of 0.5 pounds of corn sugar and 0.5 pounds of dry malt extract may have created another problem: phenols and esters. Specifically the addition of corn sugar could be the culprit for why there are hints of clove (phenolics) and banana (isoamyl acetate) in the aroma and taste. John Palmer says in How to Brew, that adding sucrose or refined sugar (corn sugar probably meets that criterion) contributes to ester production. The easily digestible sugar could have revved the English yeast (Fermentis’s Safale 04) and they could have been over-stimulated and giving off unwanted phenols and esters.

Given these modest, though real, imperfections, would we throw the whole batch down the drain, as Dogfish Head Brewing did in nearly every episode of Brew Masters?

Sam Calagione has much higher standards than we do. His company has flavor profiles and everything. Whereas our motto is "When in doubt, hop the bejeezus out of it."

Well, we here out Flog This Dead Brewery looked at our flavor profiles and realized we have no flavor profiles. We wondered if our degrees Plato were met, and we had no idea what that meant. Finally, we checked our standards, and realized we had none, well, maybe not none, but extremely low.

So, since we have incredibly low standards (after all, we answer to no one but ourselves) and we have won a gold medal for a previous screw-up, we crossed our fingers and dry-hopped the holy bejeezus out of it with 2.33 oz of cascade hops, 1 oz of simcoe hops, and 0.5 oz of Columbus hops.

This Week on Brew Disasters: Cloning Lagunitas Brewing’s Little Sumpin’ Sumpin’ Ale

Basic beer brewing equipment. Includes four fe...

Nothing like my brewing equipment (Photo credit: Wikipedia)

This Week on Brew Disasters: Cloning Lagunitas Brewing’s Little Sumpin’ Sumpin’ Ale.

Note: a pictorial of the brewing process is toward the end of this post.

We here at Flog-This-Dead Brewing are excited to try brewing a new (to us anyway) beer: Lagunitas Brewing’s Little Sumpin’ Sumpin’ Ale. The grain bill is 16 pounds; a 50/50 blend of wheat and barley. The beer has minimal brewing hops and, true to the Lagunita’s way, massive dry hopping, 4.5 ounces for a 5 gallon batch.

A huge thank you to the Jamil Zainasheff and Mike “Tasty” McDole of the Brewing Network‘s Can You Brew It and Lagunitas Brewing Company for sharing their Little Sumpin’ Sumpin’ Ale recipe with them. And, lastly, thanks to my buddy Paul for pointing me to the recipe and my loving wife for helping at just the right times.

As you may recall, we here at Flog-This-Dead Brewing just supplemented our temperature monitoring from a handheld Taylor 9842 Commercial Waterproof Digital Thermometer to a BrewMometer by Blichmann Engineering. So we started the strike water temperature for our mash at 20F above our desired mash temp, because we had found that after adding our grain the water cooled 20F. Apparently, having a thermometer probe further down makes a big difference and the mash temperature did not drop the 20F expected. Yikes, instead of a desired mash temp of 150, the mash temp was 170F! We yanked the bag of grain out of the mash tun and began cooling the watery wort down.

After cooling the mash conversion process started at a temperature of 149.5 and after 60 minutes finished at 137F. A batch sparge was used and the total wort produced was 10.5 gallons with a pre-boil specific gravity of 1.043 (1.033 @ 117F); a full 12 points below the 1.055 that was the target, the addition gallon produced had a lot to do with that (You would think I could subtract 9 from the yield of the first runnings, but no, I muffed it). One-half pound of corn sugar and one-half pound of dry malt extract were added to boost the specific gravity.

It took over about an hour and a half to get the wort to boiling for the 90 minute boil.

Total time for brewing day (including cleanup–that’s why it’s a hobby and not a business): 12 hours.

First impressions:

  • Appearance: 10L hazy-like smoggy LA day
  • Aroma: fruity, sweet,
  • Flavor: Sweet-malty, fruity, a sweetness lingers on the tip of the tongue
  • Mouthfeel: syrupy (it is wort after all)
  • Overall impression: Good start. Slightly maltier than hoppy.

The Flog This Dead Brewing‘s recipe:

Target Wort Volume Before Boil:  8.00 US gals  Actual Wort Volume Before Boil:  10.50 US gals
Target Wort Volume After Boil:  6.00 US gals  Actual Wort Volume After Boil:  7.50 US gals
Target Volume Transferred:  5.25 US gals  Actual Volume Transferred:  5.50 US gals
Target Volume At Pitching:  5.25 US gals  Actual Volume At Pitching:  5.50 US gals
Target Volume Of Finished Beer:  5.00 US gals  Actual Volume Of Finished Beer:  5.00 US gals
Target Pre-Boil Gravity:  1.053 SG  Actual Pre-Boil Gravity:  1.048 SG
Target OG:  1.079 SG  Actual OG:  1.056 SG
Target FG:  1.018 SG  Actual FG:  -No Record-
Target Apparent Attenuation:: 75.50%  Actual Apparent Attenuation: 100.00%
Target ABV: 8.10%  Actual ABV: 7.40%
Target ABW: 6.30%  Actual ABW: 5.90%
Target IBU (using Tinseth):  29.9 IBU  Actual IBU:  31.8 IBU
Target Color (using Morey):  5.3 SRM  Actual Color:  5.3 SRM
Target Mash Efficiency: 70.00%  Actual Mash Efficiency: 82.30%
Target Fermentation Temp:  64 degF  Actual Fermentation Temp:
Fermentables
Ingredient Amount % MCU When
2-Row Malt  8lb 2oz 47.30% 2.4  In Mash/Steeped
White Wheat Malt  6lb 2oz 35.60% 2.5  In Mash/Steeped
Torrified Wheat  1lb 12oz 10.20% 0.6  In Mash/Steeped
Toasted White Wheat  3.12 oz 1.10% 0.5  In Mash/Steeped
Extract – Light Dried Malt Extract  8.00 oz 2.90% 0.3  Start Of Boil
Sugar – Corn Sugar/Dextrose (Dry)  8.00 oz 2.90% 0  Start Of Boil
Hops
Variety Alpha Amount IBU Form When
Nugget 13.00%  0.39 oz 15.9  Loose Pellet Hops  90 Min From End
Willamette (rhymes with damn it) 5.20%  0.28 oz 3.9  Loose Pellet Hops  45 Min From End
Tettnanger 4.50%  1.18 oz 7.7  Loose Pellet Hops  15 Min From End
Willamette 5.20%  0.32 oz 2.4  Loose Pellet Hops  15 Min From End
Cascade 5.90%  0.85 oz 0  Loose Pellet Hops  Dry-Hopped
Centennial 9.50%  0.85 oz 0  Loose Pellet Hops  Dry-Hopped
Chinook 11.50%  0.85 oz 0  Loose Pellet Hops  Dry-Hopped
Simcoe 12.50%  0.85 oz 0  Loose Pellet Hops  Dry-Hopped
Amarillo 8.50%  0.63 oz 0  Loose Pellet Hops  Dry-Hopped
Columbus 15.50%  0.53 oz 0  Loose Pellet Hops  Dry-Hopped
Other Ingredients
Ingredient Amount When
Irish Moss  1.00 oz  in boil
Yeast
DCL S-04-SafAle

BrewingTV Reviews Heretic Brewing’s Evil Cousin

JZ on Mayhem

JZ on Mayhem (Photo credit: epicbeer)

It is great to see that Jamil Zainasheff‘s Heretic Brewing’s beers are now finding their way out onto store shelves. In this BrewingTV video Jake Keeler and Michael Dawson from Northern Brewer review Heretic Brewing’s Evil Cousin.

What they say makes me want to scour the countryside looking for this (West Coast) Imperial India Pale Ale (BJCP style 14c).