Required for yeast ranching:
- wort with a specific gravity of 1.030-1.040
- A pressure cooker (which will get up to ~235F)
- isopropyl alcohol (70)
- Petri dishes (these are cheap)
- inoculation loop
- inoculation needle
- Saline solution ( the type for contact lenses is sterile and it’s cheap)
- some known yeast to put on the petri dish
Sprinkle agar over the top of the cooled wort. Do not dump the agar rather sprinkle in around (7g/250ml). No need to stir. Sanitation is not critical yet, since it will go into a pressure cooker which serves as an autoclave. Be sure to add nutrient to your wort.
After it has been used, every time you touch something in the pressure cooker you spray it with isopropyl 70 alcohol.
Pour the agar & wort solution using the “pacman” technique
Make sure the agar sets up ~45min before flipping over to lessen the condensation
Place a drop of diluted yeast solution on the dish with the agar. Then remember the spot and drop sterile saline on the yeast’s spot.
Then use the needle to drag the drop to streak it.
Nice to haves:
- Hemocytometer (local veterinary offices may have these lying around)
To check yeast viability a bulb flask is used (9 ml of sterile and 1 ml of yeast and 1 drop of methylene blue) and .0001 ml of of solution is placed on hemocytometer slide
- Yeast: The Practical Guide to by Chris White & Jamil Zainasheff
- The Practical Brewer : a manual for the brewing industry by Harold M. Broderick, Master Brewers Association of the Americas
- Ungrateful yeast (52beers.wordpress.com)
- Brew Your Own Beer (answers.com)
- This Week in Brew Disasters: Big Irish American Red Ale follow-up/Taste and Specific Gravity (52beers.wordpress.com)
- How to Homebrew Beer (answers.com)
- This week on Brew Disasters: House Pale Ale (52beers.wordpress.com)